Next Generation Sequencing

Next- Generation DNA sequencing Review Article By SITA DAHAL postpone of contents Table of Contents2 Abstract:3 Introduction:3 Different sequencing Technologies and Data accumulation:4 SEQUENCING BY subtraction:4 SEQUENCING BY HYBRIDIZATION AND LIGATION:4 SINGLE shred SEQUENCING:5 Assembly of Huge Volume of Data from NGS:7 Applications:7 Discussion:7 Conclusion:9 even out of reference list:10 Abstract: The topic, DNA sequencing has been at hype from the remnant two decades. This review tries to unravel the divers(a) facet of this engineering and its wide range of applications not moreover in biomedical research but also in another(prenominal) argonas. at that place have been rapid technological advancements do most of the broadcast obsolete. At present, second generation sequencers ar being replaced by third generation sequencers having tenacious orders. The grandeur of bioinformatics and various other questions like where will such long amount of info lead us be dealt. We read by this lease that completion of human genome sequencing was not the finish but bonnie a starting point to individualise medicine.

Genome sequencing has helped to develop new-sprung(prenominal) vaccines and the drastic reduction in cost of sequencing weapons platform has make it possible to be used as clinical symptomatic instrument in near future. Introduction: In 1977, Fredrick Sanger demonstrable the first sequencing technique known as ambit margin order. This procedure utilizes dideoxynucleoside triphosphates (ddTTP) which are labeled with radioactive phosphate. The incorporation of ddTTP (lack! s 3 OH group) terminates the template directed synthesis of the complementary strand. The strands are denatured, size fractioned on four different lanes and the base sequences are read by auto radiography (Metzker, M.L., 2010). Using this procedure, bacteriophage ?X 174 was first sequenced genome. It is a less error-prone method and produces long reads of approximately 700 bases but endorses various drawbacks like...If you want to pass a full essay, order it on our website: BestEssayCheap.com

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